The invention relates to the expansion of T cells and particularly, although not exclusively, to the expansion of gamma delta T cells, and the optimization of medium, serum and cytokine combinations for large-scaleex vivoexpansion of gamma delta T cells for clinical use.本發明係有關於T細胞的擴展,而且特別地,雖非專有地,係有關於γδ T細胞(gamma delta T cells)的擴展,以及有關於供臨床使用的γδ T細胞之大規模離體擴展(ex vivoexpansion)的培養基、血清和細胞激素組合之最佳化。