CHOI, YUN JAIE,최윤재,KIM, HYUNG GEE,김형기,YIN JINLONG,윤금룡,JIN XUN,KIM, JUN KYUM,김준겸
申请号：
KR1020110031979
公开号：
KR1020120114468A
申请日:
2011.04.07
申请国别(地区):
KR
年份:
2012
代理人:
摘要：
PURPOSE: A mesenchymal stem cell in which endostatin and carboxyl esterase are concurrently over expressed and preventing, treating or recurrence suppression method of glioma by using the same are provided to drastically prevent carcinogenesis and recurrence.CONSTITUTION: In a mesenchymal stem cell, endostatin and carboxyl esterase are concurrently over expressed. A preventing, treating or recurrence suppression method of glioma includes a step of injecting the mesenchymal stem cell to all mammals except for human. A manufacturing method of human amniotic fluid-derived mesenchymal stem cell(AF-MSC) comprises the following steps: establishing positive number of primary cell cultivation in a minimum essential medium in which fetal bovine serum(ES-FBS) having 15% of embryonic stem cell, 2mM L-glutamine and 1% of penicillin streptomycin are contained, and 18% Chang culture medium B and 2% Chang culture medium C are supplemented collecting cells by trypsinization for selective cultivation of AF-MSC plating in DMEM low dextrose broth supplemented with 10% of FBS, 1% of penicillin streptomycin, 2mM L-glutamine and 4ng/mL basic fibroblast growth factor (bFGF: R&D) and obtaining morphologic interspecies population of the AF-MSC after second round of the subculture.COPYRIGHT KIPO 2013본 발명은 암조직으로 특이적으로 이동가능한 인간 간엽줄기세포에 엔도스타틴과 카복실에스터라아제를 발현시킨 후에 이들을 뇌종양 환자에게 주입함으로써 신경교종 형성 및 재발을 효과적으로 억제하는 방법에 관한 것으로, 본 방법에 의해 간엽줄기세포를 매개체로 엔도스타틴 및 sCE2를 암조직으로 전달하였을 때 암 형성 및 재발이 현저하게 억제되었다.
