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ACTIVITY ASSAY FOR BOND FORMING ENZYMES
专利权人:
Hoffmann-La Roche Inc.
发明人:
Mara Boenitz-Dulat,Erhard Kopetzki,Peter Kratzsch,Martin Schatte
申请号:
US15625950
公开号:
US20170370913A1
申请日:
2017.06.16
申请国别(地区):
US
年份:
2017
代理人:
摘要:
Herein is reported a method for the detection of a sortase in a sample, comprising the following steps:a) incubating the sample with a first substrate comprising an immobilization tag and a second substrate comprising a detectable label, whereby in the presence of a sortase in the sample a conjugate comprising the immobilization tag and the detectable label is formed,b) immobilizing the conjugate of step a) via/using the immobilization tag to a solid phase,c) detecting the immobilized conjugate via/using the detectable labeland thereby detecting the sortase in the sample.
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