There is described xeno-free methods, devices and systems for isolating cells from adipose tissue, the method comprising: a) providing a sample of adipose tissue b) optionally, evaluating the sample to ensure it complies with pre-determined criteria c) optionally, mincing the sample into smaller pieces d) washing the sample with a decontamination agent e) dissociating the matrix of the adipose tissue sample using a matrix dissociating agent f) isolating the nucleated cells g) purifying the nucleated cells and h) optionally, evaluating the cells of the sample to ensure they comply with pre-determined criteria, wherein the method is carried out such that no non-human biological components are introduced into the sample, and wherein isolated cells are produced. Also described are isolated cells produced by the above method.
