A nebulizer assembly includes a nebulizer host and a main flow-guiding element. The main flow-guiding element is disposed on the nebulizer host. The main flow-guiding element has a main flow-guiding channel and at least one main bottom flow-guiding opening communicating with the main flow-guiding channel, and the main bottom flow-guiding opening penetrates a bottom portion of the main flow-guiding element. A nebulized fluid generated by the nebulizer host can be sent to the main flow-guiding channel of the main flow-guiding element, and an outside air can be introduced into the main flow-guiding channel by passing through the main bottom flow-guiding opening. Therefore, the nebulized fluid in the main flow-guiding channel can be propelled towards a direction away from the at least one main bottom flow-guiding opening by the outside air flowing into the main flow-guiding channel through the main bottom flow-guiding opening.