DE COPPI, Paolo,URBANI, Luca,PINZANI, Massimo,MAZZA, Guiseppe,CROWLEY, Claire
申请号：
WO2018EP64475
公开号：
WO2018220179(A1)
申请日:
2018.06.01
申请国别(地区):
世界知识产权组织国际局
年份:
2018
代理人:
摘要：
The invention provides methods and materials for the cryopreservation of cellularised scaffolds, which may be used for therapeutic or pharmacological testing purposes, which methods comprise: (i) providing a cultured scaffold on which cells have been seeded; (ii) equilibrating said cellularised scaffold with a cryopreservative composition comprising culture medium and between 5 and 30% of a cryoprotectant such as DMSO; (iii) freezing the equilibrated cellularised scaffold by reducing the temperature continuously by about - 1°C/minute to about -80°C; (iv) storing the frozen cellularised scaffold at a temperature of between -135°C and -198°C.
