A multi-specific antibody contains at least one binding site for a cell specific antigen and at least one binding site for a tumor-reactive lymphocyte antigen and a multi-specific antibody including an IgG antibody or fragment thereof that binds to a first antigen； and at least one scFv antibody that binds to a second antigen that is different from the first antigen and is linked to a C terminus of at least one light chain or heavy chain of said IgG antibody or fragment. The multi-specific antibody reversibly binds to at least one of the cell specific antigen and tumor-reactive lymphocyte antigen or the first antigen and the second antigen with a greater affinity at an aberrant condition than at a normal physiological condition. Conjugates of the multi-specific antibodies and methods for generating the multi-specific antibody are also provided.本發明係關於一種多重特異性抗體，其含有細胞特異性抗原之至少一個結合位點及腫瘤反應性淋巴細胞抗原之至少一個結合位點，且多重特異性抗體包括結合至第一抗原的IgG抗體或其片段；及至少一種scFv抗體，其結合至不同於該第一抗原的第二抗原且連接至該IgG抗體或片段之至少一個輕鏈或重鏈的C端。該多重特異性抗體在異常條件下以比在正常生理條件下要高的親和力可逆地結合至該細胞特異性抗原及該腫瘤反應性淋巴細胞抗原之至少一者或該第一抗原及該第二抗原之至少一者。亦提供該等多重特異性抗體之共軛物及用於產生該多重特異性抗體的方法。
