Methods, systems and kits for production of autologous blood products are provided. Such methods, systems and kits comprise a blood collection set, a serum dilution set, and an aliquot set. The blood collection set includes a collection container and a serum container. The serum dilution set includes a serum measurement device, a serum dilution bag, and a mixing device enabling serum to be mixed with balanced saline solution in a defined dilution ratio. The aliquot set includes at least one filter and aliquot tubing having periodic segment length markings.
