#$%^&*AU2018200282A120180201.pdf#####ABSTRACT System and methods for stimulating cartilage formation at a first tissue site through a second tissue site is presented. The system (100) includes a fluid source (110) for supplying a therapeutic solution (112), a reduced pressure source (120) for supplying reduced pressure, a fluid delivery manifold (102) for deploying adjacent the first tissue site, and a vacuum manifold (104) for deploying within the second tissue site. The fluid delivery manifold extends between a proximal end fluidly coupled to the fluid supply and a distal end having at least one aperture for delivering the therapeutic solution to the defect adjacent the articulating surface of the first tissue site. The vacuum manifold extends between a proximal end fluidly coupled to the reduced pressure source and a distal end having at least one aperture for delivering the reduced pressure to the first tissue site adjacent the opposing surface of the first tissue site. FIG. 1>；: 010 C)N CCN CD C ICDN Co: .. 0 000 oc co0 -m --- C
