The present invention relates to the field of peanut plantation, in particular to a rhizobox device for study on peanut root morphology and use method thereof. The rhizobox device mainly comprises a box body, a bottom case, a perforated plate, and a PVC pipe, the device wholly presents a root on 5 the perforated plate, can observe, photograph and sample the whole or different levels of the root to study the morphology, structure and hierarchy thereof, solves the problems of labor-consuming, time-consuming and poor operability in existing conventional profile root digging and picking, and has the characteristics of strong operability, reusability, movability, and labor saving. Furthermore, a use method thereof is also provided. 10
