#$%^&*AU2020101557A420200903.pdf#####ABSTRACT The present invention belongs to the technical field of maize cultivation and provides a conditioner for improving high temperature resistance of maize and a preparation method and use thereof. Every 100 mL of a conditioner aqueous solution includes: 0.5-4 g of fulvic acid with a purity >; 96%, 0.5-2 g of chitosan with a purity >; 96%, 5-15 g of choline chloride with a purity >; 75%, 1-5 g of ethephon with a purity >; 80%, 0.1-1 g of acetylsalicylic acid with a purity >; 96% and 0.1-1 g of borax with a purity >; 96%. Every 100 g of a conditioner powder includes: 5-10 g of dipotassium glycyrrhizinate with a purity >; 98%, 0.1-2 g of vitamin B complex with a purity >; 96%, 40-50 g of anhydrous potassium dihydrogen phosphate with a purity >; 98%, 20-30 g of anhydrous zinc sulfate with a purity >; 96%, 1-5 g of anhydrous molybdenum sulfate with a purity >; 96% and anhydrous magnesium sulfate as balance. The conditioner can significantly improve the high temperature resistance of the maize.