A kind of urine measurement box with non-return mechanism, including ontology and check (non-return) valve; Ontology has cavity and offers the liquid inlet of connection cavity; Check (non-return) valve is arranged at what liquid inlet and in accommodating what cavity; It is wherein flowed into from liquid inlet by urine to drive check (non-return) valve to open liquid inlet, and urine then drives check (non-return) valve closing liquid entrance from cavity adverse current; Thereby, to prevent the urine adverse current in cavity from going out to measure outside box.一種具有止逆機制的尿液量測盒,包括本體及止逆閥;本體具有容腔及開設有連通容腔的液體入口;止逆閥設置於液體入口處且容置於容腔內;其中藉由尿液自液體入口流入以驅動止逆閥開啟液體入口,又尿液自容腔逆流則驅動止逆閥關閉液體入口;藉此,以防止容腔內的尿液逆流出量測盒外。