An isolated dsRNA molecule comprising an antisense RNA sequence for regulating a target gene of interest in a plant or a phytopathogen of the plant, wherein the dsRNA sequence is flanked by two complementary sites to an smRNA or smRNAs expressed in the plant and wherein the dsRNA molecule further comprises a helicase binding site positioned so as to allow unwinding of the strands of the isolated dsRNA molecule to single stranded RNA (ssRNA) and recruitment of an RNA-dependent RNA polymerase so as to amplify the dsRNA molecule in the plant cell and generate secondary siRNA products of the dsRNA sequence.SE DA A CONOCER UN MÉTODO PARA INTRODUCIR EN UNA SEMILLA UNA MOLÉCULA DE POLINUCLEÓTIDO EXÓGENO NO TRANSCRIBIBLE, POR EJEMPLO dsRNA. EL MÉTODO COMPRENDE PONER EN CONTACTO LA SEMILLA CON UNA MOLÉCULA DE POLINUCLEÓTIDO DISPARADOR EXÓGENO NO TRANSCRIBIBLE, POR EJEMPLO, dsRNA, BAJO CONDICIONES QUE PERMITEN LA PENETRACIÓN EN LA SEMILLA DE LA MOLÉCULA DE POLINUCLEÓTIDO EXÓGENO NO TRANSCRIBIBLE.
