Antiseptic compositions comprising at least one salt of EDTA are disclosed.These compositions have broad spectrum antimicrobial and antifungal activitytogether with anticoagulant properties. The antiseptic compositions also havedemonstrated activity in penetrating and breaking down microbial slime, orbiofilms. They are safe for human and medical uses, and may be used to preventinfection, or to reduce the proliferation of and/or eliminate existing orestablished infections.