The invention relates to an anti-microbial fabric impregnated with a conjugate of silver nano particles and Aloe vera (AgNP+Aloe) colloidal solution having anti-microbial property and size of 15-20nm made by synthesizing 2.5 mL of 30% ammonia solution was added to 5 mL of 10-2 M AgNO3 solution followed by addition of 5 Ml of the Aloe vera extract and a method of producing antimicrobial silver nano-particle and Aloe vera conjugated multi-purpose nano-fabric. The said method comprises steps of: a) preparation of Aloe vera gel extract and preparing required dilution with pure water b) addition of silver nitrate stock to gel extract of step (a) for synthesis of silver nano particles (SNP) c) visual observation of color change to confirm completion of reaction in step (b) d) selecting desired shape of spherical monodispersed colloidal SNP-aloe having more active facets (electron dense facets) and strongest biocidal action and size of silver nanoparticles ranges from 20 40 nm. e) making visual, spectrophotometric & XRD characterization of synthesized SNP- aloe for synthesis of silver nanoparticles confirmed by its yellow colour formation f) confirming the formation of silver nanoparticles by UV-Vis spectrophotometric characterization of SNP- aloe g) coating SNP- aloe on desired fabric such as fabrics coated are cotton (a plant derived fabric), silk (an animal fabric) and polyester (a synthetic fabric). REFERENCE FIGURE 12