NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY;SCREEN HOLDINGS CO., LTD.
发明人:
WATANABE, Mamoru,FUJII, Satoru,OKAMOTO, Ryuichi
申请号：
WO2018JP15761
公开号：
WO2018194036(A1)
申请日:
2018.04.16
申请国别(地区):
世界知识产权组织国际局
年份:
2018
代理人:
摘要：
The present invention enables easy evaluation of the water secretion function of an epithelial cell in a short time without involving staining with fluorescent pigment or the like. This method comprises: a preliminary culture step for seeding, in a culture vessel, organoids (700) established from epithelial cells and culturing the organoids in a gel (610) within each well (510) of the culture vessel for a prescribed period of time; a photographing step for taking, as a multi-pixel image, an image of each of the wells (510) containing the organoids (700); a detection step for detecting, as an object, a region, of the taken image, which is surrounded by edges and has a prescribed difference or larger in pixel intensity from the background thereof; a calculation step for calculating the area of said object inside the edges; a sorting step for sorting said object on the basis of whether or not the object is derived from the organoids; and a determination step for determining whether or not said object is to be an
