PROBLEM TO BE SOLVED: To provide a method of producing RUNX2-negative and OSX-positive osteoprogenitor cell which performs the adhesion culture of an embryoid body-derived single cell formed from a pluripotent stem cell in the presence of bFGF and TGF.SOLUTION: The adhesion culture of an embryoid body-derived single cell formed from a pluripotent stem cell is performed in the presence of bFGF and TGF. The adhesion culture of the single cell may be performed in the presence of bFGF, TGF and IGF.COPYRIGHT: (C)2016,JPO&INPIT【課題】多能性幹細胞から形成された胚様体由来の単細胞をbFGF及びTGFの存在下で接着培養する、RUNX2陰性、OSX陽性である骨前駆細胞の製造方法。【解決手段】多能性幹細胞から形成された胚様体由来の単細胞をbFGF及びTGFの存在下で接着培養する。前記単細胞をbFGF、TGFおよびIGFの存在下で接着培養してもよい。【選択図】なし
