MACDONALD LYNN,STEVENS SEAN,GURER CAGAN,MURPHY ANDREW J.,MEAGHER KAROLINA A.
申请号：
NZ70719811
公开号：
NZ707198A
申请日:
2011.06.22
申请国别(地区):
NZ
年份:
2016
代理人:
摘要：
Disclosed is a method for cloning a &lambda； (lambda) light chain variable region nucleic acid sequence, comprising the steps of: (a) obtaining a cell from a mouse comprising an endogenous &lambda； light chain allele comprising an unrearranged human V&lambda； gene segment and an unrearranged human J&lambda； gene segment, where the mouse comprises a deletion of a first V&lambda；-J&lambda；-C&lambda； gene cluster of the endogenous mouse &lambda； light chain allele and replacement, in whole or in part, of the V&lambda；-J&lambda； gene segments of a second gene cluster of the allele with the human V&lambda； and J&lambda； gene segments which are operably linked to a mouse &lambda； constant region (C&lambda；) gene so that the mouse expresses a light chain comprising a human &lambda； variable domain and a mouse C&lambda； domain； and (b) cloning from the cell a &lambda； light chain variable region nucleic acid sequence derived from the endogenous &lambda； light chain allele.
