The present invention provides a process of in situ preparation of vitamin D and vitamin D enriched nutritious kernels and applications thereof. The process comprises subjecting the variety of kernels, mixtures or parts thereof to solid state fermentation using edible and/or consumable cultures of basidiomycota and or ascomycota and exposing the fermented kernels to source of UV light. The kernels enriched with vitamin D may be used fresh or after processing for the preparation of variety of vitamin D enriched food items such as popcorns, waffle and pancake mix, breads, sweets, baked items and cookies. The kernels enriched in vitamin D are also used in nutritional enrichment in the form of flour, paste or extracts, oils or whole kernels as such.
