isolated fusion protein, homodimer, isolated homodimeric protein, isolated dimer, nucleic acid sequence, isolated polynucleotide, isolated recombinant DNA molecule, pharmaceutical composition, method for producing a protein, and, use of a fusion protein and a dimer
fusion protein, dimeric protein construction, dimeric protein, pharmaceutical composition, nucleic acid sequence, recombinant DNA molecule, cell line, methods for producing a protein and for stimulating erythrogenesis in a mammal, and, dimer a fusion protein recombinant comprising a portion of human erythropoietin peptide linked to an immunoglobulin peptide portion is described. the fusion protein has a prolonged half-life in vivo compared to naturally occurring or naturally occurring human erythropoietin. in one embodiment of the invention, the protein has an in vivo half-life at least three times that of native human erythropoietin. the fusion protein also demonstrates improved erythropoietic bioactivity compared to native human erythropoietin. in one embodiment, the fusion protein comprises the peptide sequence of an entire human erythropoietin molecule (epo) and the peptide sequence of a human immunoglobulin fc fragment igg 1. the fc fragment in the fusion protein includes the articulation, ch2 and c113 domains of human immunoglobulin iggl. the epo molecule can be linked directly to the fc fragment to avoid foreign peptide linkers and decrease the risk of an immunogenic response when administered in vivo. in one embodiment, the hinge region is a variant of the human fc fragment having a non-cysteine residue in amino acid 6. the invention also relates to the nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods to produce the fusion protein. the invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and / or the pharmaceutical compositions, for example to stimulate erythropoiesis in individuals in need of therapy.proteina de fusão, construção de proteina dimerica, proteina dimerica, composição farmacêutica, sequencia de ácido nucleico, molécula de dna recombinante, linhagem de células, metodos para produzir uma proteina e para estimular a eritr
