Disclosed is a traditional Chinese medicine composition for protecting the liver and kidneys and a preparation method and use thereof. The composition is composed of 100-200 parts of Polygonatum odoratum, 100-200 parts of fructus lycii, 80-160 parts of radix ophiopogonis, 70-130 parts of Angelica sinensis, 70-130 parts of semen cassiae, 70-130 parts of white peony root, 70-130 parts of dried orange peel, 70-130 parts of prepared rehmannia root, 70-130 parts of chrysanthemum, 30-70 parts of rhizoma chuanxiong, 20-40 parts of irkutsk anemone rhizome, and 10-20 parts of bupleurum by weight.