A nucleotide construct comprising a nucleotide sequence that forms a stem anda loop, wherein the loop comprises a nucleotide sequence that modulates expressionof a target, wherein the stem comprises a nucleotide sequence that modulatesexpression of a target, and wherein the target modulated by the nucleotide sequencein the loop and the target modulated by the nucleotide sequence in the stem maybe the same or different. Vectors, methods of regulating target expression,methods of providing a cell, and methods of treating conditions comprising thenucleotide sequence are also disclosed.
