MANETTI, ANDREA,CAPO, SABRINA,BENSI, GIULIANO,GRANDI, GUIDO,MARGARIT Y ROS, IMMA
申请号：
NZ59163309
公开号：
NZ591633A
申请日:
2009.09.17
申请国别(地区):
NZ
年份:
2012
代理人:
摘要：
Disclosed are compositions useful for reducing the risk of, preventing, and/or treating S. pyogenes (GAS) infections which comprise combinations of GAS antigens, nucleic acid molecules encoding the antigens, or antibodies which specifically bind to the antigens. The compositions comprise: (a) a combination of three or more different Streptococcus. pyogenes (GAS) protein antigens selected from the group consisting of: (1) Spy0167 or a mutant Spy0167 protein having a hemolytic activity that is reduced by at least 50% relative to a wild-type Spy0167 protein, wherein said mutant Sp0167 protein comprises one or more amino acid substitutions or deletions at one or more amino acid positions selected from the group consisting of P427, W535, C530, A248, and D482 numbered according to the sequence set forth in SEQ ID NO: 107； (2) Spy0269； and (3) Spy0416 or a mutant Spy0416 protein having a proteolytic activity against interleukin-8 (IL-8) as determined by SDS-polyacrylamide gel electrophoresis or ELISA that is reduced by at least 50% relative to a wild-type Spy0416 protein, wherein said mutant Sp0416 protein comprises one or more amino acid substitutions or deletions at one or more amino acid positions selected from the group consisting of D151, H279, and 5617 numbered according to the sequence set forth in SEQ ID NO: 1； or (b) one or more nucleic acid molecules encoding the GAS protein antigens at (1), (2), and (3)； or (c) three or more different antibodies, wherein each antibody selectively binds to a GAS protein antigen selected from the group consisting of (1), (2), and (3), wherein each GAS protein antigen is different and the antibodies do not cross-react with each other.
