Recombinational lucid ganoderma immunomodulatory protein methoxypolyethyleneglycol propionic acid succinimidyl ester modifier, preparation method and use
Disclosed are a recombinational lucid ganoderma immunomodulatory protein methoxypolyethyleneglycol propionic acid succinimidyl ester modifier, preparation method and use. Disclosed is recombinational lucid ganoderma immunomodulatory protein methoxypolyethyleneglycol propionic acid succinimidyl ester modifier the preparation method of the modifier is: to the reaction system of 0.1 Mu pEta5.0-pH8.0 phosphate buffer, feeding the rLZ-8 dimer and the mPEG-SPA at the mole ratio of 1 : 1-1 : 6, stirring the mixture by magnetic force at room temperature, reacting same for 1.0-2.5 hours in the dark, and obtaining a modifier with a purity of up to 98% after a purification and the modifier is used in the preparation of medicine for treating leucopenia caused by the chemotherapeutic drugs. The effects of the present invention consist in that the steps of the preparation method are simple and the product is single the half life of the modifier in vivo is significantly lengthened compared with that of the pre-modified rLZ-8 (see FIG. 2) the lowest dosage of the administration and the onset time in the study of treating leucopenia are both better than that of the pre-modified rLZ-8.一種重組靈芝免疫調節蛋白rLZ-8的單甲氧基聚乙二醇丙酸琥珀醯亞胺酯修飾物、製備方法和用途;其中該修飾物製備方法:在0.1M pH 5.0-pH 8.0磷酸鹽緩衝液的反應體系中將rLZ-8二聚體與mPEG-SPA以摩爾比為1:1-1:6進行投料,室溫下磁力攪拌,避光條件下反應1.0-2.5小時,經純化得到純度達到98%的修飾產物;該修飾物在製備治療化療藥所致白細胞減少症的藥物中的應用;本發明效果在於製備方法步驟簡單,產物單一;又該修飾物其體內半衰期較修飾前rLZ-8出現顯著延長;且在治療白細胞減少症研究中的最低給藥劑量和起效時間均優於修飾前rLZ-8。
