Chung Yuan Christian University;Academia Sinica;National Defense Medical Center
发明人:
Yu-Chan CHAO,Nenavath Gopal NAIK,Tzong-Yuan WU,Chang-Chi LIN,Szu-Cheng KUO
申请号:
US15992178
公开号:
US20190365852A1
申请日:
2018.05.30
申请国别(地区):
US
年份:
2019
代理人:
摘要:
Disclosed herein are recombinant baculoviruses suitable for introducing an exogenous gene into a pest insect, particularly, disease-transmitting mosquitos. The recombinant baculovirus is characterized in having a promotor that is any of a HzNV-1 viral early expressing gene pag1, a ceropin gene b1, a defensin gene a4, or hp70 gene; and an exogenous gene operably linked thereto the promoter. Also disclosed herein is a method of introducing an exogenous gene into a pest insect. The method includes transducing the pest insect with a recombinant baculovirus without suppressing the production of microRNAs (miRNAs) in the pest insect, wherein the recombinant baculovirus comprises a promoter of pag1, cecropin b1, defensin gene a, or hp70.