對單核苷酸ＫＲＡＳ突變具專一性之雙功能性短髮夾ＲＮＡ（ＢＩ

发明人:: RAO, DONALD,劳　唐诺,勞　唐諾,WANG, ZHAOHUI,王朝晖,王朝暉,NEMUNAITIS, JOHN J.,奈穆纳提司　约翰Ｊ,奈穆納提司　約翰Ｊ,SENZER, NEIL,杉瑟尔　尼尔,杉瑟爾　尼爾,劳唐诺,勞唐諾,奈穆纳提司约翰J,奈穆納提司約翰J,杉瑟尔尼尔,杉瑟爾尼爾

摘要：: The present invention includes compositions and methods for making and using a bifunctional shRNAs capable of reducing an expression of a K-ras gene, eg., a mutated K-ras gene, wherein at least one target site sequence of the bifunctional RNA molecule is located within the K-ras gene and wherein the bifunctional RNA molecule is capable of activating a cleavage-dependent and a cleavage-independent RNA-induced silencing complex for reducing the expression level of K-ras.本發明包括一種製造及使用能夠減少K-ras基因(例如突變K-ras基因)表現之雙功能性shRNA之組合物及方法，其中該雙功能性RNA分子之至少一個靶位點序列係位於該K-ras基因內，且其中該雙功能性RNA分子能夠活化裂解依賴型及非裂解依賴型RNA誘導之沉默複合物，以減少K-ras之表現量。