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PROCEDES DE PRODUCTION DE CONJUGUES DE FACTEURS DE CROISSANCE I DE TYPE INSULINE (IGF-I) ET DE POLYETHYLENE GLYCOL
专利权人:
F.HOFFMANN-LA ROCHE AG
发明人:
申请号:
EP07801961.9
公开号:
EP2059261B1
申请日:
2007.08.29
申请国别(地区):
EP
年份:
2012
代理人:
摘要:
The present invention relates to a fusion protein comprising IGF-I or an IGF-I variant N-terminally linked to the C-terminus of a propeptide. The invention relates also to a method involving the use of the aforementioned fusion protein in the production of a lysine-PEGylated IGF-I or IGF-I variant. The method comprises the steps of cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding the fusion protein and causing the cell to express the fusion protein, recovering and PEGylating said fusion protein, cleaving said PEGylated fusion protein with IgA protease, and recovering lysine-PEGylated IGF-I or IGF-I variant. The invention relates also to a lysine-PEGylated IGF-I or IGF-I variant produced using the above method. In addition, the invention relates to a method for treating a neurodegenerative disorders like Alzheimer's Disease using the lysine-PEGylated IGF-I or IGF-I variant and a composition comprising the lysine-PEGylated IGF-I or IGF-I variant.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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