The present invention relates to prime boost regimens that involve the administration of at least one mRNA construct such as the use of such constructs in boost administration subsequently to prime administration of certain other antigenic composition(s). Such inventive regimens may in particular be useful for the induction of an immune response in a subject and/or the vaccination of such subject against infection from one or more pathogens and/or the treatment or prevention of one or more diseases or conditions including a tumour or cancer allergy or autoimmune conditions and/or a disease or condition associated with infection from a pathogen. The present invention further describes methods uses vaccination compositions kits and packaged vaccine components related to or useful for one or more of such regimens.
