전북대학교산학협력단;주식회사 중앙백신연구소;CHOONG ANG VACCINE LAB.;INDUSTRIAL COOPERATION FOUNDATION CHONBUK NATIONAL UNIVERSITY;주식회사 중앙백신연구소;전북대학교산학협력단
发明人:
김원일,윤인중,최환원,이승철,노윤희,이동욱,KIM WON IL,YOON IN JOONG,CHOI HWAN WON,LEE SEUNG CHUL,NOH YUN HEE,LEE DONG UK
申请号：
KR1020180146829
公开号：
KR1020200061484A
申请日:
2018.11.24
申请国别(地区):
KR
年份:
2020
代理人:
摘要：
In the present invention, a PCV-2d pathogenicity evaluation experiment was performed using SPF pigs. As a result, as a clinical symptom, after observation for 3 weeks after inoculation, 1 of 2 out of 3 test groups died on the 19th day after inoculation, and in the remaining subjects, clinical symptoms of PMWS, wasting, lethargy, and roughness were observed. In addition, in viremia and virus excretion, during the test period, viremia continued to rise up to 21 days after inoculation, showing a titer of up to 10 3.95±1.46 TCID 50 /ml and nasal cavity up to 10 4.72±0.77 TCID 50 /ml (vaccinated After 14 days), fecal up to 10 4.81±0.53 TCID 50 /ml (14 days after inoculation) showed a virus release pattern. In addition, as a result of evaluating the virus in the tissue, more than 10 4 TCID 50 /ml of virus was detected in all organs. In addition, as a result of the antibody test, no positive conversion was observed even after 3 weeks of inoculation. In addition, in the results of gross lesions and histological examination, the lymph nodes were observed at the time of autopsy, and lymphoid depletion and lymphoid depletion, which are necessary and sufficient conditions for PMWS, and histiocytic changes in Lymphoid tissue and PCV-2 antigen detection of the corresponding organs were also confirmed (average score 2.15). ) It became. In addition, vascular/peribronchial inflammatory cell infiltration, acute lesions of purulent bronchial pneumonia and interstitial pneumonia due to secondary infection after PCV-2 infection, was observed in the lung. Hepatitis and hepatic necrosis were also observed, and hepatic icterus due to liver lesions and ascites due to portal hypertension were observed.본 발명에서, SPF 돼지를 이용하여 PCV-2d 병원성 평가실험을 수행하였다. 그 결과, 임상증상으로서, 접종 후 3주간 관찰 결과, 시험군 3두 중 접종 후 19일차에 1두가 폐사하였고 나머지 개체의 경우 PMWS의 임상증상인 wasting, 무기력, 피모거침 등이 관찰되었다. 또한, 바이러스 혈증 및 바이러스 배출에서, 시험기간 동안 바이러스 혈증은 접종 후 21일까지 지속적으로 상승하여 최대 103.95±1.46 TCID50/ml의 역가를 보였고 비강으로 최대 104.72±0.77 TCID50/㎖ (접종 14일 후
