The present invention provides a method for assessingernbryotoxicity of a chemical comprising: (1) a first step ofmeasuring the expression level of one or more genes selected fromamong genes each comprising any of the nucleotide sequences of SEQID NOs: 1 to 78 and 101 to 230 and orthologous genes thereof ina sample from a non-human mammal or mammalian cell which has comeinto contact with a test chemical； and (2) a second step of comparingthe measured value of the expression level of the gene in the sampleobtained in the first step with a control value of the expressionlevel of the gene and based on the difference assessing the levelof the embryotoxicity of the test chemical in the sample； and soon.
