The subject invention concerns new classes of insecticidally-active proteins and the polynucleotide sequences which encode these proteins. More specifically, insecticidal proteins of approximately 12-24 kDa and of approximately 12-14 kDa are used for controlling corn rootworms. The subject invention includes methods and transgenic plants for controlling Western Corn Rootworm and other coleopteran insects.本發明涉及新類別的殺蟲活性蛋白以及編碼這些蛋白的聚核苷酸序列。更具體而言,約12-24kDa及約12-14kDa的殺蟲蛋白係使用來防治玉米根蟲。本發明包含用於防治西方玉米根蟲與其他鞘翅目昆蟲之方法及基因轉殖植物。