A puncture device comprising a puncture device and a socket. The puncture device comprises a base and a puncture portion, and the puncture portion includes a protrusion and a tip end, and the protrusion extends radially outward from the outer surface of the puncture portion. The socket includes a pneumatic seat, a first cannula assembly, a second cannula assembly, a mount, and an air bag. The second cannula assembly is retractable to receive the first cannula assembly in the second cannula assembly. The fixing seat is fixed to the second sleeve assembly, and the inner wall of the fixing seat has a guiding groove and a key groove. The guiding groove has a depth greater than the key groove, and the guiding groove communicates with the key groove. When the protruding block of the puncture device is entered by the guiding groove, After the rotation of the bump to set the key groove, the puncture device is fixed to the fixing seat, and the puncture device drives the fixing seat to move along a long axis direction of the puncture device.一種穿刺器，包含穿刺器械及套接件。穿刺器械包含底座及穿刺部，且穿刺部包凸塊及尖端，凸塊由穿刺部的外表面向外徑向延伸。套接件包含氣壓座、第一套管組件、第二套管組件、固定座、以及氣囊。第二套管組件可收縮，使第一套管組件容置於第二套管組件中。固定座固定於第二套管組件，且固定座的內壁具有導引槽及鍵槽，導引槽的深度大於鍵槽，導引槽與鍵槽連通，當穿刺器械之凸塊由導引槽進入，經旋轉使得凸塊卡設鍵槽後，穿刺器械與固定座固定，穿刺器械帶動固定座沿穿刺器械的一長軸方向上移動。
