Disclosed is a composition containing active components of Dracocephalum moldavica L. against myocardial ischemia-reperfusion injury (MIRI). The composition consists of active components and a pharmaceutically acceptable carrier, wherein the active components comprise luteolin, kaempferol and luteolin-7-0-glucoside. Pharmacodynamic experiments show that these three components can be combined together to synergistically treat myocardial ischemia-reperfusion injury.