A disposable catheterization device includes a guiding member and two connection member. The guiding member has a guiding portion, a first open end and a second open end. The guiding portion is consisted of two side walls. The bottom edge of the side walls is a closed structure. The first open end is disposed opposite to the second open end, which are located at two ends of the guiding portion. Each connection members is extended from the guiding member and located between the first open end and the second open end.