Disclosed is a surgical tool guider which is easily installed and separated to shorten an operation time and is prevented from deviating from an abdominal wall and free from a danger of leaking gas, thereby allowing an operation to be performed smoothly and stably. A protection cap for a surgical tool guider is provided to move in conjunction with a surgical tool while maintaining the sealing state even when the surgical tool is variously moved for an operation. Thus, leakage of gas is effectively prevented and the operation is performed smoothly without an inconvenience of injecting gas again during the operation.