The embodiments herein provide a therapeutic composition comprising N (delta)-protonated L-2-amino-5-diaminomethyleneamino-pentanoic acid called J Factor and a method of ex-vivo synthesis of J-Factor from N(omega)′-protonated L-arginine at a pH of 5-9. The method involves increasing a temperature and or decreasing an acidity of an aqueous solution containing the N(omega)′-protonated L-arginine to obtain a pH value of greater than pKa−2 or less than pKa+2. The derivatives of aqueous solution are allowed to reach an equilibrium state at 25° C. The acidity of the acidity of the aqueous solution is adjusted to a pH value of 7.0 at 25° C. The pKa is a minus logarithm of an acid dissociation constant of the N(omega)′-protonated guanidino group. The N(omega)′-protonated 1-arginine includes ionized forms in the carboxyl or 2-amino group, which are in equilibrium with the N(omega)′-protonated 1-arginine. The composition has a molecular mass of more than or equal to 175.2u
