There is provided a seedbed holding unit capable of reliably and firmly holding a seedbed with a simple structure, causing no damage to a plant. A seedbed holding unit 160 that holds a seedbed B used in plant cultivation includes a first holding member 170 and a second holding member 180 that are rotatable relative to each other about a predetermined rotation axis A, the first holding member 170 is provided with a first claw part 173 in a fixed state, the second holding member 180 is provided with a second claw part 183 in a fixed state, and the seedbed B is held between the first claw part 173 and the second claw part 183 by rotating the first holding member 170 and the second holding member 180 relative to each other.