The present invention relates to a novel bacteriophage ΦCJ27 (KCCM11465P) and a composition containing the same as an active ingredient. Further, the present invention relates to a method for preventing and/or treating infective diseases caused by enterotoxic Escherichia coli (ETEC) of non-human animals using the ΦCJ27 (KCCM11465P) and the composition.