Provided herein are methods of applying the ERH gene in the preparation of bladder cancer diagnosis and treatment products. The ERH gene is found to have a good correlation with bladder cancer. In addition, an RNA interference vector is constructed to perform cell function experiments to study the effect of ERH gene on the proliferation and clonality of bladder cancer cells. The methods disclosed herein provide a research basis for clinical diagnosis and treatment of bladder cancer and has a good application prospect.