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VACCINES BASED ON A NON-INTEGRABLE RETROVIRAL VECTOR
专利权人:
ЛЕНТИГЕН КОРПОРЕЙШЕН (US)
发明人:
ДРОПУЛИК Боро (US)
申请号:
RU2012140691/10
公开号:
RU2012140691A
申请日:
2010.03.13
申请国别(地区):
RU
年份:
2014
代理人:
摘要:
1. Non-integrating non-replicating lentiviral vector containing a long terminal repeat, the sequence necessary for packaging and a heterologous promoter functionally linked to one or more polynucleotide sequences that together encode the structural proteins of the virus. 2. The vector according to claim 1, wherein the structural proteins themselves assemble into a virus-like particle (VLP) when polynucleotide sequences are expressed in a cell transduced by the vector. The vector according to claim 2, containing a self-inactivating (SIN) vector. The vector according to claim 1 or 2, in which the virus is selected from the group consisting of lentivirus, influenza virus, hepatitis virus, alphavirus, filovirus and flavivirus. A vector according to claim 1 or 2, wherein the structural proteins include a virus capsid protein. A vector according to claim 1 or 2, wherein the structural proteins include a virus envelope protein. A vector according to claim 1 or 2, further comprising a heterologous polynucleotide sequence that encodes an immunostimulatory protein. The vector according to claim 1 or 2, containing a heterologous polynucleotide sequence that encodes a bacterial, viral or tumor antigen. The vector according to claim 1 or 2, wherein the structural proteins are selected from the group consisting of HIV gag protein, influenza matrix protein and hepatitis virus core protein. The vector of claim 8, wherein the heterologous env gene is selected from the group consisting of env VSV-G gene, influenza A virus env gene, influenza B virus env gene, hepatitis C virus env gene, Ebola virus env gene, Ebola virus env gene, causing the "Marburg disease", and the dengue virus env gene. 11. The use of lentiviral vector according to any one of1. Неинтегрирующийся нереплицирующийся лентивирусный вектор, содержащий длинный концевой повтор, необходимую для упаковки последовательность и гетерологичный промотор, функционально связанный с одной или несколькими полинуклеотидными последов
来源网站:
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