MOLECULE D'ADN CIRCULAIRE COMPRENANT UNE ORIGINE DE REPLICATION CONDITIONNELLE, PROCEDE DE PREPARATION DE CETTE MOLECULE ET UTILISATION CORRESPONDANTE DANS LA THERAPIE GENIQUE
A prokaryotic recombinant host cell comprising a heterologous replicationinitiation protein that activates a conditional origin of replication and anextrachromosomal DNA molecule comprising a heterologous therapeutic gene and aconditional origin of replication whose functionality in the prokaryoticrecombinant host cell requires a replication initiating protein which isforeign to the host cell is described. The host cell may comprise a pir genehaving at least one mutation, which may occur in the pir gene copy numbercontrol region, the pir gene leucine zipper-like motif, or the pir gene DNAbinding region.
