The present invention relates to a production method of tolerogenic plasmacytoid dendritic cells, tolerogenic plasmacytoid dendritic cells produced by the method, and, cell therapeutic agents including the same. In the method, the plasmacytoid dendritic cells with immune tolerance may be induced from the immature dendritic cells at a high yield using a simple and easy process, thereby enabling a stable supply of large amounts of tolerogenic plasmacytoid dendritic cells.