Method for genetic inactivation of unfertilized puffer eggs, method for producing super male pufferfish having sex chromosome YY, and method for producing male pufferfish using the same
<；P>；PROBLEM TO BE SOLVED: To provide a technique capable of surely obtaining supermale globefishes having YY sex chromosomes and thereby finally and surely producing only the male globefishes. <；P>；SOLUTION: The super male globefishes 11 having YY sex chromosomes can be obtained through the steps of radiation exposure for genetically inactivating eggs by applying a radiation dose 3 of 50 to 800 Gy to the unfertilized eggs 1 of the globefish, fertilizing the genetically inactivated unfertilized eggs 4 with sperm 6 of a normal male globefish 5, and giving heat shock 8 to haploid embryos 7 to block the second segmentation, and performing the polyploidization of the chromosome. By mating the super male globefish 11 with a normal female globefish 12, only the male globefishes 15 can be produced. As the globefish, Takifugu rubripes or Takifugu porphyreus is preferable. <；P>；COPYRIGHT: (C)2011,JPO&INPIT
