PROTEÍNAS DE FUSÃO COMPREENDENDO UMA PROTEASE NÃO CITOTÓXICA, UMA UNIDADE DE DIRECCIONAMENTO, UM LOCAL DE CLIVAGEM DE PROTEASE E UM DOMÍNIO DE TRANSLOCAÇÃO
KEITH FOSTER,JOHN CHADDOCK,PHILIP MARKS,PATRICK STANCOMBE,LYNDSEY DUROSE
申请号：
PT05818352
公开号：
PT1874807E
申请日:
2005.12.01
申请国别(地区):
PT
年份:
2013
代理人:
摘要：
The invention provides a single chain, polypeptide fusion protein, comprising: a non-cytotoxic protease, or a fragment thereof, which protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of a target cell； a Targeting Moiety that is capable of binding to a Binding Site on the target cell, which Binding Site is capable of undergoing endocytosis to be incorporated into an endosome within the target cell； a protease cleavage site at which site the fusion protein is cleavable by a protease, wherein the protease cleavage site is located between the non-cytotoxic protease or fragment thereof and the Targeting Moiety； and a translocation domain that is capable of translocating the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the target cell.
