JAMAL SABIR M. SABIR,AHMED ATEF BIOMY,FOTOUH M. EL-DOIATY,SHERIF EDRIS,NAHID H. HAJRAH,OSAMA A. ABUZINADAH,AHMED BAHIELDIN
申请号:
US14983448
公开号:
US20170182188A1
申请日:
2015.12.29
申请国别(地区):
US
年份:
2017
代理人:
摘要:
A method for preparing chitosan-coated magnetic nanoparticles for protein immobilization includes forming ferrous ferric oxide (Fe3O4) nanoparticles by co-precipitation and coating the nanoparticles with chitosan in the presence of glutaraldehyde. The Fe3O4 nanoparticles can be coated by dispersing ferrous ferric oxide (Fe3O4) nanoparticles into a solution comprising chitosan and acetic acid, adding a surfactant, adding excess 50% glutaraldehyde solution, and washing the nanoparticles with a solvent. The chitosan coated ferric oxide (Fe3O4) nanoparticles can be used to immobilize proteins or other biomolecules.