The present invention provides a cartilage matrix having a high decellularization level arid a low glycosaminoglycan (GAG) content. The cartilage matrix exhibits desirable characteristics, for example, cohesiveness, tackiness and malleability, for use in cartilage repair. Also provided is a method of preparing the cartilage matrix, comprising decellularizing a cartilage to generate a decellularized cartilage, and deglycosylating the decellularized cartilage.