The present invention regards a sterile workspace with removable layers for use on medical surfaces such as anesthesia workstations, as well as other surfaces. The assembly of the present invention generally includes a plurality of sterile workspaces, layered to form the assembly. Each workspace has a foam layer and a barrier layer. In some configurations, the workspace also has a tray, and the foam layer is secured to the top surface of the tray. The tray may have a raised border about its perimeter. In some configurations the foam layer has a non-porous bottom surface. The assembly may have a support surface under the bottom-most workspace layer, with an elastomer or adhesive affixed thereto to prevent the assembly from moving during use. The barrier layer is affixed to either the foam layer, or the tray (if present), and is removed to expose a sterile workspace when in use.
