Lee, Mi Yeon,Jeon, Hong Bae,Yang, Yoon-Sun,Oh, Won Il,Kwon, Sun Jae
申请号：
AU2013309642
公开号：
AU2013309642B2
申请日:
2013.09.02
申请国别(地区):
AU
年份:
2018
代理人:
摘要：
Disclosed is a method for culturing mesenchymal stem cells, comprising culturing mesenchymal stem cells in a medium containing calcium in a concentration of from 2.1 to 3.8 mM and magnesium in a concentration of from 1.0 to 3.0 mM under a hypoxic condition of 2 to 5 % oxygen. The culturing method can increase the population of mesenchymal stem cells even with a small number of passages by improving mesenchymal stem cells in proliferative capacity and viability. In addition, the mesenchymal stem cells prepared by the culturing method are effectively used not only as a safe cell therapeutic agent due to their lacking immunogenicity, but also as a cartilage regenerating medicine owing to their excellent secretion of cytokines.
