The present invention provides methods of treating, protecting against, and inducing an immune response against a human papillomavirus-associated tumor or cancer, comprising the step of administering to a subject a recombinant Listeria strain expressing a construct comprising at least one human papillomavirus antigen.本發明提供了針對人類乳突狀瘤病毒相關的腫瘤或癌症之治療、預防及誘發免疫反應的方法,其包括對一個體投與一重組李斯特菌菌株的步驟,該重組李斯特菌菌株係表現一包括有至少一人類乳突狀瘤病毒抗原的構築體。