Mary K. Estes,David Graham,Robert Legare Atmar,Sue Ellen Crawford,Khalil Ettayebi,Kosuke Murakami
申请号：
US15763695
公开号：
US20180282704A1
申请日:
2016.10.03
申请国别(地区):
US
年份:
2018
代理人:
摘要：
Embodiments of the disclosure concern systems, methods, and/or compositions for cultivation of mammalian viruses, including at least human noroviruses and sapoviruses within the Caliciviridae family of viruses. The ex vivo culture systems include intestinal enteroids in combination with bile or a functionally active fraction or component thereof. In specific embodiments, the culture system is utilized to test inactivation compounds for therapeutic or environmental efficacy and to test contaminated comestibles and/or environmental entities for determination of the presence of infectious virus. Furthermore, antiviral compositions may be tested using systems of the disclosure, including drugs, small molecule inhibitors, and biologies such as neutralizing monoclonal antibodies.
